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2024年5月16日发(作者:)

PodA:LeicaStellaris5

LaserScanningConfocalMicroscope

KyleMarchukAustinEdwardsMohammadNaserHarrisonWismer

May2023

Contents

1Introduction

2Start-Up

3FocusingYourSample

4InitialImageSetup

.

5ImagingParameters

6ImagingModules

7SavingAcquiredImages

8OtherImagingTips

9ShutDown

1

2

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3

6

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14

1Introduction

TheLeicaStellaris5isaninvertedlaserscanningconfocalwith4laserlines(405nm,488nm,561nm,and638

nm).Itfeatures3next-generationHybridDetectors(HyDs).

AllnewusersneedtobetrainedbyaBIDCstaffmemberbeforeindependentuse.

SignupfortimeusingtheiLabmicroscopescheduler.

TheobjectivesavailableforuseontheStellaris5are:

Objective

HC

HC

HC

HC

HC

PLFluotar

PLAPOCS2

FluotarVISIR

PLAPOCS2

PLAPO

Mag

5x

20x

25x

40x

63x

Numerical

Aperture

0.15

0.75

0.95

1.30

1.40

Immersion

Air

Air

Water

Oil

Oil

Working

Distance

13.7mm

0.62mm

2.40mm

0.24mm

0.14mm

IDNumber

506224

506517

506375

506358

506350

2Start-Up

ure:1.

NOTE:ThekeyshouldalwaysbeOnandthemicroscopepowerboxshouldalwaysbeOn.

Figure1:Thepowersupplyswitcheslocatedtotheleftofthecomputerdesk.

thecomputertower.

2

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