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2024年3月12日发(作者：)

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专利名称：METHODS OF LOWERING THE ERROR RATE

OF MASSIVELY PARALLEL DNA SEQUENCING

USING DUPLEX CONSENSUS SEQUENCING

发明人：Michael Schmitt,Jesse Salk,Lawrence A. Loeb

申请号：US16120091

申请日：20180831

公开号：US2A1

公开日：20181220

摘要：Next Generation DNA sequencing promises to revolutionize clinical medicine

and basic research. However, while this technology has the capacity to generate

hundreds of billions of nucleotides of DNA sequence in a single experiment, the error

rate of approximately 1% results in hundreds of millions of sequencing mistakes. These

scattered errors can be tolerated in some applications but become extremely

problematic when “deep sequencing” genetically heterogeneous mixtures, such as

tumors or mixed microbial populations. To overcome limitations in sequencing accuracy, a

method Duplex Consensus Sequencing (DCS) is provided. This approach greatly reduces

errors by independently tagging and sequencing each of the two strands of a DNA

duplex. As the two strands are complementary, true mutations are found at the same

position in both strands. In contrast, PCR or sequencing errors will result in errors in only

one strand. This method uniquely capitalizes on the redundant information stored in

double-stranded DNA, thus overcoming technical limitations of prior methods utilizing

data from only one of the two strands.

申请人：UNIVERSITY OF WASHINGTON THROUGH ITS CENTER FOR

COMMERCIALIZATION

地址：Seattle WA US

国籍：US

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