产铁载体菌筛选和对藻类生长影响地研究

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2024年5月16日发(作者：)

华 中 科 技 大 学 硕 士 学 位 论 文

growth rate, the concentration of Chlorophyll a, carotenoids, total soluble proteins,

phycocyanin and microcystin were detected. According to the data obtained ,we found that

addition of siderophore have a negative impact on the growth of cyanobacteria samples, the

decline of the cell density (OD680) and the cell number of the growth rate show that the

growth rate of the cyanobacteriasampls were suppressed. In the experimental stage,

cyanobacteriasampls of FACHB-905 experimental group1, 2, 3 were 66.18%, 77.27%,

FACHB-245 experimental group1, 2, 3 were 66.18%, 77.27%, 82.32 % of the control

group. At the same time, the chelation of siderophore inhibit absorption of Fe

3+

for

cyanobacteria, affect synthesis of chlorophyll and protein so that the cyanobacteria die,

inhibit the production of cyanotoxins in M. aeruginosa. Microcystin of FACHB-905

experimental group1, 2, 3 were 28.54%, 41.59%, 50.73% of the control group. The result

show that siderophores of high-yielding siderophore bacteria could be used as an effective

means for controlling cyanobacterial overgrowth such as M. aeruginosa and A.

flos-anabaena in eutrophic waterbodies.

Key words: Siderophores Identification of bacteria Orthogonal test method

M.

aeruginosa

A. flos-anabaena

IV

华 中 科 技 大 学 硕 士 学 位 论 文

目 录

摘 要 ............................................................................................................... I

Abstract .......................................................................................................... III

1 绪论

1.1 铁及铁载体........................................................................................... (1)

1.2 铁及铁载体与蓝藻生长的关系 .......................................................... (5)

1.3 国内外研究现状 .................................................................................. (7)

1.4 本文的研究内容 .................................................................................. (8)

2 产铁载体菌的筛选及生化指标的鉴定

2.1 引言 ..................................................................................................... (10)

2.2 实验材料与方法 ................................................................................ (10)

2.3 结果与讨论......................................................................................... (14)

2.4 本章小结 ............................................................................................. (22)

3 系统发育树的构建及序列分析

3.1 引言 ..................................................................................................... (23)

3.2 实验材料与方法 ................................................................................ (23)

3.3 结果与讨论......................................................................................... (28)

3.4 本章小结 ............................................................................................. (32)

4 产铁载体量的测定及培养条件的优化

4.1 引言 ..................................................................................................... (33)

4.2 实验材料与方法 ................................................................................ (33)

V

本文标签： 生长筛选研究